1
Ezaacelial" laccase (E.C. 1.10.3.2) from the culture filtrate of
Purified by ammovme male Prectpltatioa, protamse sulfate 10 phadex w~
ion exchange cbromatop~apby and Sephadex G100 Precipitation, DEAE-SePha A-SO
weight of the enzyme was estimated by SDS.poiyacryla d de dUOesis to be T~ 58,000 and the fsoeiecMc point was 3.75. The t esb 51,00 and th hO was 6.5. The optimum temperature for the enzyme was about 45°C and the optimum
enzyme was found to be stable at temperature below 35°C and rapidly ~w temperatures. Em values for ferul c add, vanilWc add, dihydron (DOlaactiA) were
41.6μM, O.S2mM, and 2.73 mM, respectively, which indicates that the enzyme s much w afflu .
ty towards terulic sue. The reaction products of the enzyme were ~Yme ya C a Hp LC.
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